CELT-426

Tocris Bioscience | Catalog # 7955

Potent and partially selective hD2 dopamine receptor fluorescent antagonist
Tocris Bioscience

Key Product Details

Description

Potent and partially selective hD2 dopamine receptor fluorescent antagonist

Wavelength

Excitation = 560 nm, Emission = 571 nm
View all Fluorescent Receptor Probes »

Product Description

CELT-426 is a potent and partially selective fluorescent hD2 dopamine receptor antagonist (Ki values are 89.3 nM, 194.8 nM, and 263.6 nM for D2, D3, and D4 dopamine receptors respectively in radioligand binding assay). CELT-426 can be used as a fluorescent dopamine receptor probe for fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It is a suitable replacement for GPCR radiolabeling ligands. Excitation and emission maxima (λ) are 560 and 571 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

Add 62 μL of assay buffer containing 1% of DMSO to obtain a 100 μM stock solution, and use 250 nM as working concentration.

Scientific Data Images for CELT-426

Application of CELT-426 in high content screening assay.

Application of CELT-426 in high content screening assay.

Competition binding assay between CELT-426 (100 nM) and increasing concentrations of haloperidol (Catalog # 0931) in live adherent CHO cells expressing hD2.

Application of CELT-426 in fluorescence polarization assay.

Application of CELT-426 in fluorescence polarization assay.

Human dopamine D2 receptor was isolated from CHO cells by membrane preparation and incubated with CELT-426 (100 nM). Haloperidol (Catalog # 0931) was added across a range of concentrations, and the binding affinity (KD = 3 nM) determined by fluorescence polarization.

Licensing Information

Sold under license from Celtarys Research

Optical Data

Emission Color Yellow
λabs 560 nm
λem 571 nm
Closest Laser line 561 nm
Application Fluorescence polarization, Confocal microscopy, Flow Cytometry, High-content screening,

Spectra Viewer

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Product Specifications for CELT-426

Molecular Weight

1615.85

Storage

Store at -20°C

Purity

≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

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Product Documents for CELT-426

Certificate of Analysis

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Product Specific Notices for CELT-426

For research use only

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