ch-TOG Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92555

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ch-TOG Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1). LLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ch-TOG Antibody - BSA Free

Western Blot: ch-TOG AntibodyBSA Free [NBP2-92555]

Western Blot: ch-TOG AntibodyBSA Free [NBP2-92555]

Western Blot: ch-TOG Antibody [NBP2-92555] - Analysis of extracts of various cell lines, using ch-TOG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP2-92555] - Immunofluorescence analysis of Mouse L929 cells using ch-TOG antibody (NBP2-92555) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP2-92555] - Immunofluorescence analysis of U2OS cells using ch-TOG antibody (NBP2-92555) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP2-92555] - Immunofluorescence analysis of Rat H9C2 cells using ch-TOG antibody (NBP2-92555) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunoprecipitation: ch-TOG Antibody - BSA Free [NBP2-92555]

Immunoprecipitation: ch-TOG Antibody [NBP2-92555] - Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug ch-TOG antibody (NBP2-92555). Western blot was performed from the immunoprecipitate using ch-TOG antibody (NBP2-92555) at a dilution of 1:1000.

Applications for ch-TOG Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ch-TOG

ch-TOG, also known as CKAP-5, is a microtubule-associated protein that is involved in microtubule dynamics required for mitotic spindle formation. Specifically, chTOG protects kinetochore microtubules from depolymerization and plays an important role in centrosomal microtubule assembly. ch-TOG is typically overexpressed in tumor cells, may therefore be associated with cancer development.

ch-TOG antibodies are useful tools for cytosleletal research and for some cancer studies.

Alternate Names

CHTOG, Ch-TOG, Colonic and hepatic tumor over-expressed gene protein, cytoskeleton associated protein 5, cytoskeleton-associated protein 5, EC 2.7.7.6, EC 3.1.1.29, KIAA0097FLJ35359, MSPS, TOG, TOGp

Gene Symbol

CKAP5

Additional ch-TOG Products

Product Documents for ch-TOG Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ch-TOG Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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