Cholesterol

Key Product Details

Product Description

Cholesterol is a major sterol in all mammalian organisms; it is an essential component that makes up about 20-25% of the structural lipids of cell membranes. Cholesterol determines the permeability and fluidity characteristics of the cell membranes. Cholesterol is an endogenous ligand for estrogen-related receptor α (ERRα). It is also a precursor in several biosynthetic pathways, including steroid hormones and the active form of vitamin D. Cholesterol is a component of lipid nanoparticles (LNPs), and is important in modulating membrane stability, with the effect being context dependent. When combined with lipids that have low gel-liquid crystalline phase transition temperature (T_m), Cholesterol reduces membrane fluidity and increases bilayer thickness; when combined with high T_m lipids, it increases membrane fluidity and narrows the bilayer thickness.

For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.

For a fully formulate LNP containing DLin-MC3-DMA and a luciferase mRNA reporter, see MC3 LNP Luciferase (Cat. No. 8889).

Product Specifications for Cholesterol

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Cholesterol

The following data is based on the product molecular weight 386.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.59 mL	12.93 mL	25.86 mL
5 mM	0.52 mL	2.59 mL	5.17 mL
10 mM	0.26 mL	1.29 mL	2.59 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 21 publications citing the usage of this product.

• Cheng Dendrimer-based lipid nanoparticles deliver therapeutic fah mRNA to normalize liver function and extend survival in a mouse model of hepatorenal tyrosinemia type I. Adv.Mater 2018 PMID: 30368954
• Ghanbari Cholesterol as an endogenous ligand of ERRα promotes ERRα-mediated cellular proliferation and metabolic target gene expression in breast cancer cells. Cell 2020 PMID: 32717915
• Tenchov Lipid nanoparticles - from liposomes to mRNA vaccine delivery, a landscape of research diversity and advancement. ACS Nano 2021 PMID: 34181394

Product Documents for Cholesterol