CLASP1 Antibody (KT66) - C-terminus - BSA Free

Novus Biologicals | Catalog # NBP3-23219

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # KT66

Format

BSA Free
View all CLASP1 Primary Antibodies »

Product Specifications

Immunogen

Clone KT66 was generated from a GST fusion protein containing the C-terminus of mouse CLASP1. This sequence is highly conserved in human, rat, chicken, and fish CLASP1, and has low homology to CLASP2. [UniProt# Q80TV8]

Specificity

The antibody detects a 161 kDa protein corresponding to the molecular mass of CLASP1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP1 by immunocytochemistry in HeLa cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for CLASP1 Antibody (KT66) - C-terminus - BSA Free

CLASP1 Antibody (KT66)

Western Blot: CLASP1 Antibody (KT66) [NBP3-23219] -

Western Blot: CLASP1 Antibody (KT66) [NBP3-23219] - Western blot of CLASP1 in mouse brain (lane 1) and rat PC12 cells (lane 2). The blots were probed with rat monoclonal NBP3-23219 anti-CLASP1 (C-terminus) at a dilution of 1:500. Then detected using donkey anti-Rat IgG:HRP.

Applications for CLASP1 Antibody (KT66) - C-terminus - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLASP1

CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM]

Alternate Names

CLIP-associating protein 1, cytoplasmic linker associated protein 1, Cytoplasmic linker-associated protein 1, DKFZp686D1968, DKFZp686H2039, hOrbit1, KIAA0622FLJ33821, MAST1FLJ41222, MGC131895, multiple asters 1, Multiple asters homolog 1, Protein Orbit homolog 1

Gene Symbol

CLASP1

Additional CLASP1 Products

Product Documents for CLASP1 Antibody (KT66) - C-terminus - BSA Free

Certificate of Analysis

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Product Specific Notices for CLASP1 Antibody (KT66) - C-terminus - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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