Clathrin light chain Antibody (CON.1) - BSA Free

Novus Biologicals | Catalog # NB600-1520

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CON.1

Format

BSA Free
View all Clathrin light chain Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide, corresponding to amino acids 23-44 of Cow Clathrin Light Chain.

Reactivity Notes

Cross-reacts S. cerevisiae. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cell Membrane and Cytoplasmic

Specificity

Monoclonal Anti-Clathrin Light Chain reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. The product is useful in immunoblotting 2-4 (two doublets at 25-36 kDa, a doublet at approximately 56 kDa, and possibly also additional lower and higher molecular weight bands), immunoprecipitation, immunocytochemistry (4% paraformaldehyde- 0.5% Triton X-100 fixation), and immunoaffinity purification. Cross-reactivity has been observed with human, bovine, rat, mouse, and yeast.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Clathrin light chain Antibody (CON.1) - BSA Free

Application
Recommended Usage

Western Blot

1:250
Application Notes
WB: Detects a doublet at approximately 25-36 kDa, a doublet at approximately 56 kDa and possibly additional bands (lower and higher molecular weight).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Clathrin light chain

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Transcript Variant: This variant (1) lacks alternate in-frame exons, compared to variant 2, resulting in a shorter protein (isoform a), compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

clathrin light chain A, clathrin, light chain A, Lca, light polypeptide (Lca)

Entrez Gene IDs

1211 (Human); 12757 (Mouse); 83800 (Rat)

Gene Symbol

CLTA

Additional Clathrin light chain Products

Product Documents for Clathrin light chain Antibody (CON.1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Clathrin light chain Antibody (CON.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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