CLIC4 Antibody

Novus Biologicals | Catalog # NBP1-33474

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Zebrafish

Predicted:

Bovine (98%), Mouse (98%), Rat (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CLIC4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLIC4. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (87%).

Localization

Cytoplasm, Cytoplasmic vesicle membrane, Single-pass membrane protein, Nucleus, Cell membrane, Single-pass membrane protein, Cytoplasm, Cytoplasmic vesicle membrane, Nucleus, Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CLIC4 Antibody

Western Blot: CLIC4 Antibody [NBP1-33474]

Western Blot: CLIC4 Antibody [NBP1-33474]

Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody [NBP1-33474]

Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody [NBP1-33474]

Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-33474] - Paraformaldehyde-fixed HeLa, using CLIC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Western Blot: CLIC4 Antibody [NBP1-33474]

Western Blot: CLIC4 Antibody [NBP1-33474]

Western Blot: CLIC4 Antibody [NBP1-33474] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.
CLIC4 Antibody

Immunohistochemistry: CLIC4 Antibody [NBP1-33474] -

Immunohistochemistry: CLIC4 Antibody [NBP1-33474] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CLIC4 antibody (NBP1-33474) at 1:300 dilution.
CLIC4 Antibody

Western Blot: CLIC4 Antibody [NBP1-33474] -

Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (NBP1-33474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CLIC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CLIC4

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Alternate Names

chloride intracellular channel 4, chloride intracellular channel 4 like, chloride intracellular channel protein 4, CLIC4L, DKFZp566G223, FLJ38640, H1, huH1, Intracellular chloride ion channel protein p64H1, MTCLIC, P64H1

Gene Symbol

CLIC4

Additional CLIC4 Products

Product Documents for CLIC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CLIC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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