![Western Blot: CLIP170 Antibody [NBP2-15917]](https://resources.rndsystems.com/images/products/CLIP170-Antibody-Western-Blot-NBP2-15917-img0001.jpg "Western Blot: CLIP170 Antibody [NBP2-15917]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human CLIP170. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%).
Localization
Cytoplasm; cytoskeleton
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CLIP170 Antibody
Western Blot: CLIP170 Antibody [NBP2-15917]
Western Blot: CLIP170 Antibody [NBP2-15917] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody [NBP2-15917] -
CLIP170 antibody [N1], N-term detects CLIP170 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CLIP170 stained by CLIP170 antibody [N1], N-term (NBP2-15917) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications for CLIP170 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CLIP170
Alternate Names
CAP-GLY domain containing linker protein 1, CLIP, CLIP170, CLIP-170Reed-Sternberg intermediate filament-associated protein, CYLN1MGC131604, cytoplasmic linker 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, Reed-Steinberg cell-expressed intermediate filament-associated protein, Restin, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein), RSNCAP-Gly domain-containing linker protein 1
Gene Symbol
CLIP1
UniProt
Additional CLIP170 Products
Product Documents for CLIP170 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CLIP170 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for CLIP170 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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