Colon Matched Tumor and Normal Tissue Lysate
Novus Biologicals | Catalog # NBP2-47072
Key Product Details
Species
Product Summary for Colon Matched Tumor and Normal Tissue Lysate
1 x Human colon normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate Set
Diagnosis: AdenocarcinomaSex: Male
Age: 44
Grade: 1
Stage: II, T3N0M0
Tumor Pathology Data
Location: Colon
Gross findings: Tumor size 5 x 5 x 3 cm with ulcerative surface. Cut surface is soft, white. Regional lymph nodes: 4
Microscopic Findings
Tissue sections show proliferation of malignant epithelial cell clusters. Epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Mitotic figures are evident. The tumor cells form glands and invaded through the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are revealed. Lymph nodes show negative.
Preparation Method
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
Product Specifications
Application Notes
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Type
Life Stage
Tissue
Formulation, Preparation, and Storage
Concentration
Shipping
Storage
Product Documents for Colon Matched Tumor and Normal Tissue Lysate
Certificate of Analysis
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Product Specific Notices for Colon Matched Tumor and Normal Tissue Lysate
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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