CometAssay Analysis Software

Catalog # Availability Size / Price Qty
4260-000-CS
Product Details
Citations (6)
FAQs
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CometAssay Analysis Software Summary

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Stability & Storage
Store the unopened product at room temperature. Do not use past expiration date.

Citations for CometAssay Analysis Software

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The synthetic food dye, Red 40, causes DNA damage, causes colonic inflammation, and impacts the microbiome in mice
    Authors: Zhang, Q;Chumanevich, AA;Nguyen, I;Chumanevich, AA;Sartawi, N;Hogan, J;Khazan, M;Harris, Q;Massey, B;Chatzistamou, I;Buckhaults, PJ;Banister, CE;Wirth, M;Hebert, JR;Murphy, EA;Hofseth, LJ;
    Toxicology reports  2023-12-01
  2. Tuning ultrasmall theranostic nanoparticles for MRI contrast and radiation dose amplification
    Authors: Brown, N;Rocchi, P;Carmès, L;Guthier, R;Iyer, M;Seban, L;Morris, T;Bennett, S;Lavelle, M;Penailillo, J;Carrasco, R;Williams, C;Huynh, E;Han, Z;Kaza, E;Doussineau, T;Toprani, SM;Qin, X;Nagel, ZD;Sarosiek, KA;Hagège, A;Dufort, S;Bort, G;Lux, F;Tillement, O;Berbeco, R;
    Theranostics  2023-08-21
  3. The DNA damage response in advanced ovarian cancer: functional analysis combined with machine learning identifies signatures that correlate with chemotherapy sensitivity and patient outcome
    Authors: TDJ Walker, ZF Faraahi, MJ Price, A Hawarden, CA Waddell, B Russell, DM Jones, A McCormick, N Gavrielide, S Tyagi, LC Woodhouse, B Whalley, C Roberts, EJ Crosbie, RJ Edmondson
    British Journal of Cancer, 2023-02-21;0(0):.  2023-02-21
  4. Overcoming Temozolomide Resistance in Glioblastoma via Enhanced NAD+ Bioavailability and Inhibition of Poly-ADP-Ribose Glycohydrolase
    Authors: J Li, CA Koczor, KM Saville, F Hayat, A Beiser, S McClellan, ME Migaud, RW Sobol
    Cancers, 2022-07-22;14(15):.  2022-07-22
  5. Direct interaction of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus
    Authors: I Rashid, M Hammel, A Sverzhinsk, MS Tsai, JM Pascal, JA Tainer, AE Tomkinson
    The Journal of Biological Chemistry, 2021-06-25;0(0):100921.  2021-06-25
  6. Sensitive CometChip assay for screening potentially carcinogenic DNA adducts by trapping DNA repair intermediates.
    Authors: Ngo L, Owiti N, Swartz C, Winters J, Su Y, Ge J, Xiong A, Han J, Recio L, Samson L, Engelward B
    Nucleic Acids Res, 2020-02-20;0(0):.  2020-02-20

FAQs

  1. What image analysis tools are available for analysis of data generated by the CometAssay®, and CometChip®?

    • We offer a software (Catalog # 4260-000-CS) designed to improve, standardize, and make comet analysis easier. The software will automatically locate and score comets from digital images to characterize and quantify the degree of DNA damage revealed by the comet assay. CometAssay, and CometChip Kits. The Comet Analysis Software can rapidly evaluate large numbers of cells and generate summary statistics based on the corresponding numeric results.

  2. Is there a demo or free trial available for the CometAssay Analysis Software?

    • A video is available on the Bio-Techne website to demonstrate how the software works (Home » Resources » Protocols » How to Run the CometAssay Analysis Software). A demo or free-trial of the software is not currently available for customers to try with their own samples before purchase. 

  3. Can the CometAssay Analysis software be installed on multiple computers at the same time?

    • The software can be installed on multiple computers but the USB dongle needs to be plugged-in for the software to work. That limits it to only running the software on one computer at a time. 

  4. What type of images will the CometAssay Analysis software accept? What image magnification is acceptable?

    • The CometAssay Analysis Software will accept TIFF, JPEG or PNG images. A magnification of 4X or 10X is acceptable. 

  5. Can Comets be picked manually with the CometAssay Analysis Software?

    • No. The software automatically picks Comets to be analyzed once study parameters are defined. Comets can manually be excluded from the analysis. 

  6. Is there a recommended image bit-size for the CometAssay analysis software?

    • The maximum image size is limited by the hardware capabilities of the computer running the CometAssay software. It is recommended that one work with the highest quality of images that can be used on a particular computer without incurring significant wait times in the image import process. The original, uncompressed image files captured by the microscope imaging software will provide the best results for Comet assay analysis.

  7. What factors can affect the inclusion of Comets when using an image with the Comet Assay analysis software?

    • The quality of the image used for analysis can affect the inclusion of the Comets for analysis. If the image used for analysis has very bright Comets and/or is over-exposed, the software's dynamic range may prevent it from including dimmer Comets if they are adjacent to bright Comets. One way to get around this is to split the images into quadrants (or other pieces) so that the brightest spots are not close to the dim spots. The dimensions and resolution should not be changed when splitting images.

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