Q: What power supply is recommended for the CometAssay Electrophoresis System?

A: We recommend PowerPac™ HC High-Current Power Supply (1645052), available from Bio-Rad.

Q: Which adaptor can be used with the PowerPac ( Bio-Rad®, Catalog # 1645052) recommended for the CometAsay Electrophoresis System II?

A: The PowerPac Adator (Catalog # 1645064) can be used with the PowerPac recommended for the CometAsay Electrophoresis System II.

Q: Will any commercial horizontal gel electrophoresis device work for the Comet Assay?

A: Electrophoresis conditions described for FLARE™ Assay, CometAssay® and CometChip® Kits were optimized for the CometAssay® Electrophoresis System (Catalog # 4250-050-ES). Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). They can be used, but they require optimization by the user to achieve consistent results.

CometAssay Electrophoresis System II

R&D Systems | Catalog # 4250-050-ES

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Product Summary
Preparation & Storage
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Key Product Details

Features

Enables optimization and standardization of alkaline and neutral comet assays.

Key Benefits

Maintains constant buffer temperature with cooling pack chamber and one-piece molded plastic body.
Maintains optimal buffer level for consistent results with overlay.
Specially designed trays accommodate 2, 20 and 96 well slides and maintain correct position during electrophoresis.
Optimized for use with CometAssay Kits and CometAssay Control Cells.

Product Summary for CometAssay Electrophoresis System II

What is the CometAssay?

The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.

Why Use the CometAssay Electrophoresis System?

CometAssay ES Il enables investigators to consistently optimize alkaline and neutral comet assays for highly reproducible results, and to standardize electrophoresis methods between individual users and laboratories. Comet assay results can be variable depending on temperature, distance between electrodes, and buffer height. This specialized electrophoresis unit helps resolve these variables.

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.

Storage

Store the unopened product at room temperature. Do not use past expiration date.

Product Documents for CometAssay Electrophoresis System II

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Note: Certificate of Analysis not available for kit components.

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