Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COMP/Thrombospondin-5 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human COMP/Thrombospondin-5

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for COMP/Thrombospondin-5 Antibody - BSA Free

Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-99528]

Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-99528]

Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-99528] - Anti-COMP/Thrombospondin-5 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 84 kDa. Observed band size: 94 kDa

Applications for COMP/Thrombospondin-5 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COMP/Thrombospondin-5

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for COMP/Thrombospondin-5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for COMP/Thrombospondin-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Customer Reviews for COMP/Thrombospondin-5 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review COMP/Thrombospondin-5 Antibody - BSA Free and earn rewards!

Have you used COMP/Thrombospondin-5 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for COMP/Thrombospondin-5 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

    • Q: I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
      A: We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374
Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies

Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail