Complement C4d Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-31130

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Complement C4d Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa1241-1256 of C4 conjugated to KLH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Scientific Data Images for Complement C4d Antibody - BSA Free

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130]

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130]

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.
Immunohistochemistry: Complement C4d Antibody [NBP2-31130]

Immunohistochemistry: Complement C4d Antibody [NBP2-31130]

Immunohistochemistry: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of human kidney
Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130]

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130]

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.

Applications for Complement C4d Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:80

Immunohistochemistry-Frozen

1:20-1:80

Immunohistochemistry-Paraffin

1:20-1:80
Application Notes
Immunohistochemistry (Paraffin): Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining. Immunofluorescence. Optimal dilutions to be determined by the researcher.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.1% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement C4d

Complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved resulting in C4c and C4d, in which step all functional sites are lost. Therefore, the C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.

Alternate Names

C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, complement C4A, CPAMD2, RG

Gene Symbol

C4A

UniProt

P0C0L4 (Human)

Additional Complement C4d Products

Product Documents for Complement C4d Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Complement C4d Antibody - BSA Free

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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