CRLF3 Antibody (PSH03-88)

Western Blot: CRLF3 Antibody (PSH03-88) [NBP3-32223]

Western Blot: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: Raji cell lysate (20 ug/Lane)
Lane 3: Ramos cell lysate (20 ug/Lane)
Lane 4: K-562 cell lysate (20 ug/Lane)
Lane 5: Jurkat cell lysate (20 ug/Lane)
Lane 6: 293T cell lysate (20 ug/Lane)
Lane 7: A549 cell lysate (20 ug/Lane)
Lane 8: RAW264.7 cell lysate (20 ug/Lane)
Lane 9: PC-12 cell lysate (20 ug/Lane)
Lane 10: Mouse thymus tissue lysate (40 ug/Lane)
Lane 11: Mouse spleen tissue lysate (40 ug/Lane)
Lane 12: Rat thymus tissue lysate (40 ug/Lane)
Lane 13: Rat testis tissue lysate (40 ug/Lane)

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: CRLF3 Antibody (PSH03-88) [NBP3-32223]

Immunocytochemistry/ Immunofluorescence: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.