Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Cultrex 3-D Spheroid Colorimetric Assay, 96-well
R&D Systems | Catalog # 3511-096-K
Colorimetric Proliferation/Viability Assay
Discontinued Product
3511-096-K has been discontinued.
View all Basement Membrane Extracts products.
Key Product Details
Features
Provides a powerful tool for modeling tumor growth response in vitro using colorimetric detection.
Species
Mouse
Product Summary for Cultrex 3-D Spheroid Colorimetric Assay, 96-well
Why Use Cultrex 3-D Spheroid Colorimetric Assay, 96-well?
The Cultrex 3-D Spheroid Colorimetric Proliferation/Viability Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment. Tumor spheroid expansion is visualized microscopically and can be quantified through image analysis software for real-time and label free evaluation. At the conclusion of the assay, cell viability may be assessed by absorbance using MTT. The 3-D Spheroid Colorimetric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantitifying cell viability within the spheroids in response to pharmacological treatment.
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Kit Contents for Cultrex 3-D Spheroid Colorimetric Assay, 96-well
- Cultrex Spheroid Formation Matrix
- 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
- MTT Reagent
- Detergent Reagent
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Basement Membrane Extracts
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Product Documents for Cultrex 3-D Spheroid Colorimetric Assay, 96-well
Certificate of Analysis
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Verified Customer | Posted 04/02/2018
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Verified Customer | Posted 12/06/2016We use this to isolate stem cells and grow them in spheroid to test different experimental drugs for their efficacy to kill cancer stem cells.
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