Cultrex 3-D Spheroid Colorimetric Assay, 96-well
Cultrex 3-D Spheroid Colorimetric Assay, 96-well Summary
Why Use Cultrex 3-D Spheroid Colorimetric Assay, 96-well?
The Cultrex 3-D Spheroid Colorimetric Proliferation/Viability Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment. Tumor spheroid expansion is visualized microscopically and can be quantified through image analysis software for real-time and label free evaluation. At the conclusion of the assay, cell viability may be assessed by absorbance using MTT. The 3-D Spheroid Colorimetric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantitifying cell viability within the spheroids in response to pharmacological treatment.
• Cultrex Spheroid Formation Matrix
• 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
• MTT Reagent
• Detergent Reagent
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
For research use only. Not for diagnostic use.
Citations for Cultrex 3-D Spheroid Colorimetric Assay, 96-well
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Ablation of the ASCT2 (SLC1A5) gene encoding a neutral amino acid transporter reveals transporter plasticity and redundancy in cancer cells
Authors: A Bröer, G Gauthier-C, F Rahimi, M van Gelder, D Dorsch, A Wegener, J Holst, S Bröer
J. Biol. Chem., 2019;0(0):. 2019
An actionable axis linking NFATc2 to EZH2 controls the EMT-like program of melanoma cells
Authors: V Perotti, P Baldassari, A Molla, G Nicolini, I Bersani, G Grazia, F Benigni, A Maurichi, M Santinami, A Anichini, R Mortarini
Oncogene, 2019;0(0):. 2019
The Aged Microenvironment Influences the Tumorigenic Potential of Malignant Prostate Epithelial Cells
Authors: D Bianchi-Fr, M Damodarasa, SA Hernandez, RM Gil da Cos, F Vakar-Lope, IM Coleman, MJ Reed, PS Nelson
Mol. Cancer Res., 2019;17(1):321-331. 2019
Resistance to MAPK inhibitors in melanoma involves activation of the IGF-1R-MEK5-Erk5 pathway
Authors: L Benito-Jar, M Díaz-Martí, N Arellano-S, P Vaquero-Mo, A Esparís-Og, J Teixidó
Cancer Res., 2019;0(0):. 2019
MicroRNA-205-5p inhibits three-dimensional spheroid proliferation of ErbB2-overexpressing breast epithelial cells through direct targeting of CLCN3
Authors: T Takeno, T Hasegawa, H Hasegawa, Y Ueno, R Hamataka, A Nakajima, J Okubo, K Sato, T Sakamaki
PeerJ, 2019;7(0):e7799. 2019
Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma
Authors: JK Thompson, A Shukla, AL Leggett, PB Munson, JM Miller, MB MacPherson, SL Beuschel, HI Pass, A Shukla
Oncotarget, 2018;9(1):293-305. 2018
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We use this to isolate stem cells and grow them in spheroid to test different experimental drugs for their efficacy to kill cancer stem cells .