Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear, with Phenol Red
Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear, with Phenol Red Summary
• Ideal for general cell culture application
• Reduced growth factor formulation provides a more defined culture system
• Polymerizes at 37°C to form a reconstituted basement membrane
• Quality controlled for peformance consistency
Why Use Cultrex RGF BME?
Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. It is processed to reduce matrix-associated growth factors to provide a matrix that can be used to create a more defined model system. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex RGF BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
• Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME)
• Phenol Red
Gelling Assay - Cultrex RGF BME gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 14 days at 37 °C.
For research use only. Not for diagnositic use.
What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
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