Cultrex Stem Cell Qualified Human Fibronectin, PathClear

Fibronectin specifically qualified for use in stem cell applications
Catalog # Availability Size / Price Qty
3420-001-03
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Cultrex Stem Cell Qualified Human Fibronectin, PathClear Summary

Cultrex Stem Cell Qualified Human Fibronectin, Pathclear is a purified stromal extracellular matrix protein that provides a functionally defined and effective feeder-free surface for the attachment and maintenance of pluripotent stem cells in culture.

Key Benefits

• Qualified as a substrate for pluripotent stem cells
• Promotes a feeder-free culture
• Pathclear-tested to ensure product is free of human virus and pathogens
• Source tissue is LDEV-free

Why Use Cultrex Stem Cell Qualified Human Fibronectin?

Cultrex Stem Cell Qualified Human Fibronectin, Pathclear is purified from human plasma and is Pathclear certified to be free of common pathogens. Fibronectin is an extracellular matrix protein that is found abundantly in blood and connective tissues. These matrices are associated with the epithelial to mesenchymal transition of metastatic cells, including tumor cells with stem cell-like properties. Fibronectin performs essential functions in collagen fibrillogenesis, as either a general cell adhesion molecule or a modulator in binding between cell surfaces and the extracellular matrix. Fibronectin matrix assembly is essential for normal vertebrate development and apparently contributes to the generation of tumor metastases by supporting the establishment and persistence of pre-metastatic niches. Fibronectin is secreted as a disulfide-linked dimer of 230-270 kDa, comprised of three types of repeating modules that mediate interactions with extracellular matrix components (including fibronectin itself), and cells via integrins and other fibronectin receptors. Thus, fibronectin can be used for coating tissue culture surfaces or as a medium additive to promote stem cell adhesion and proliferation. It is 0.2 μm sterile filtered.

Specifications

Source
Human plasma
Protein Concentration
1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
Tested negative by PCR test for mycoplasma and human pathogenic viruses, including: EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, and VZV.
Stability
Product is stable for a minimum of 3 months from date of shipment when stored at ≤ -20 °C. For optimal stability store at ≤ -70 °C. Avoid freeze-thaw cycles.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Human

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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