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Cultrex Stem Cell Qualified Laminin I, Pathclear

R&D Systems | Catalog # 3400-010-03

Mouse Laminin I specifically qualified for use in stem cell applications.
R&D Systems
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Key Product Details

Features

Cultrex Stem Cell Qualified Laminin I provides a functionally defined and effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in an undifferentiated state.

Key Benefits

  • Qualified to support pluripotent stem cell expansion and differentiation
  • Pathclear-tested to ensure a pathogen and virus-free material
  • Source tissue is LDEV-free

Species

Mouse

Product Summary for Cultrex Stem Cell Qualified Laminin I, Pathclear

Why Use Cultrex Stem Cell Qualified Laminin I?
Cultrex Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. This highly purified preparation of mouse Laminin I is composed of alpha 1 beta 1 gamma 1 chains with a total molecular weight (MW) of 800 KDa.

Negative for the presence of bacteria and fungi.

Product Specifications

Source

Murine Engelbreth-Holm-Swarm (EHS) tumor

Protein Concentration

1 mg/mL

Endotoxin Level

≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay

Sterility Testing

No bacterial or fungal growth detected following 14 days in culture

Cell Culture Testing

Supports cell attachment and spreading of MG63 human osteosarcoma cells.

Viral Testing

Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Stability

Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.

Background: Laminin-1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of combinations of five known alpha, four beta and three gamma chains in mammals. One alpha, beta and gamma subunit self-assembles to form at least 15 coiled-coil heterotrimers. Through specific interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes and survival. Some subunits, such as Laminin gamma1 (Laminin B2), are present in several laminins; Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin). Others, such as the beta3 and gamma2 subunits, occur in only one known combination (e.g. with alpha3 in Laminin-5 / Epiligrin).

Alternate Names

Laminin-111, Laminin1

Additional Laminin-1 Products

Product Documents for Cultrex Stem Cell Qualified Laminin I, Pathclear

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Citations for Cultrex Stem Cell Qualified Laminin I, Pathclear

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FAQs for Cultrex Stem Cell Qualified Laminin I, Pathclear

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can Cultrex Mouse Laminin I be used on glass cover slips?

    A: Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

  • Q: What is the recommended working concentration for Cultrex Laminin I?

    A: The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.  

  • Q: Can Cultrex Mouse Laminin I be used on glass cover slips?

    A: Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

  • Q: What is the recommended working concentration for Cultrex Laminin I?

    A: The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.  

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