Cultrex Stem Cell Qualified Laminin I, Pathclear
Cultrex Stem Cell Qualified Laminin I, Pathclear Summary
• Qualified to support pluripotent stem cell expansion and differentiation
• Antibiotic-free formulation
• Pathclear-tested to ensure a pathogen and virus-free material
• Source tissue is LDEV-free
Why Use Cultrex Stem Cell Qualified Laminin I?
Cultrex Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. This highly purified preparation of mouse Laminin I is composed of alpha 1 beta 1 gamma 1 chains with a total molecular weight (MW) of 800 KDa.
For research use only. Not for diagnositic use.
Citation for Cultrex Stem Cell Qualified Laminin I, Pathclear
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Derivation of enteric neuron lineages from human pluripotent stem cells
Authors: K Barber, L Studer, F Fattahi
Nat Protoc, 2019;14(4):1261-1279. 2019
What is the recommended working concentration for Cultrex Laminin I?
The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.
What is Laminin I?
Laminin I is a major component of extracellular matrix. Cultrex Laminin I is purified from murine EHS sarcoma. It is composed of α1β1γ1 chains with a total MW of 800,000 Da. Cultrex Laminin I increases cell adhesion, migration, growth, differentiation, neurite outgrowth, protease production, and malignancy. The response is dependent on cell type.
Can Cultrex Mouse Laminin I be used on glass cover slips?
Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.
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