Best Seller

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

R&D Systems | Catalog # 3434-010-02

Basement membrane extract specifically qualified to support feeder-free stem cell culture for embryonic and pluripotent stem cells.
R&D Systems
100% Guarantee

Key Product Details

Features

Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (Catalog # 3434-010-02) is equivalent to Geltrex™ Reduced Growth Factor Basement Membrane Matrix, LDEV-Free, Stem Cell Qualified (Catalog # A1413301 and A1413302).

Key Benefits

  • Qualified to support the culture of pluripotent stem cell
  • Supports human iPSC and ESC expansion and differentiation
  • Reduced growth factor formulation provides a more defined culture system
  • Quality controlled for performance consistency

Species

Mouse

Product Summary

Why Use Cultrex Stem Cell Qualified RGF BME?
Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex Stem Cell Qualified RGF BME has been shown to provide an effective surface for feeder-free stem cell attachment and maintenance of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is useful for promoting the expansion of pluripotent stem cells or for the study of stem cell differentiation.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Product Specifications

Source

Murine Engelbreth-Holm-Swarm (EHS) tumor

Protein Concentration

8-12 mg/mL as determined by Lowry assay

Endotoxin Level

≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay

Sterility Testing

No bacterial or fungal growth detected following 14 days in culture

Cell Culture Testing

Promotes the human induced pluripotent stem cell attachment.

Effectively maintains human iPSCs in a pluripotent state in a feeder-free stem cell culture.

Viral Testing

Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Scientific Data Images

Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs).

Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs).

Human iPSCs were grown for a minimum of 2 passages on either Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02) or a leading competitor's matrix, prior to creating embryoid bodies for hematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME, showed a greater efficiency to develop into hematopoietic stem cells (CD34+, CD45+). Data courtesy of the Verneris Laboratory at the University of Colorado.
Colonies of Human iPSCs Grown on Cultrex Stem Cell Qualified RGF BME.

Colonies of iPSCs Grown on Cultrex Stem Cell Qualified RGF BME.

Human erythroblasts, reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4X magnification. Data courtesy of the Harvard Stem Cell Core.

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Stability

Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.

Background: Basement Membrane Extracts

Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo.  All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.

Additional Basement Membrane Extracts Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

Customer Reviews

There are currently no reviews for this product. Be the first to review Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract and earn rewards!

Have you used Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Cell Culture Products