Cy5B-trisulfo, NHS
Tocris Bioscience | Catalog # 7394
Key Product Details
Description
Wavelength
Product Description
Key information:Cy5B-trisulfo, NHS is a far-red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Particularly useful for smFRET experiments to reduce blinking and bleaching artefacts and maintain correct mean FRET efficiencies.
Application: FRET, smFRET, FLIM, TIRF, and super resolution microscopy (SRM) techniques, including dSTORM. Provides enhanced spatial resolution in dSTORM experiments. Does not provide efficient blinking in conventional high-thiol dSTORM buffers; instead, sodium borohydride (NaBH4) addition is required following which SMLM experiments can be performed in PBS.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 685 nm, respectively; extinction coefficient = 241,000 M-1cm-1; quantum yield = 0.4; fluorescence lifetime = 1.85 ns.
We suggest making up a 10 mM stock solution of this product in anhydrous DMSO.
For more information on how Cy5B-trisulfo, NHS may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Scientific Data Images for Cy5B-trisulfo, NHS
Application of Cy5B-trisulfo, NHS in COS7.
FLIM composite image of a COS7 cell immunostained with mouse anti-beta -tubulin primary and Cyanine 5 labelled secondary (green), and rabbit anti-clathrin primary plus Cy5B-trisulfo, NHS labelled secondary (yellow-red). Scale bars, 5 μm and 1 μm (insets). Image kindly provided by Dr. Martin Schnermann, Center for Cancer Research, National Cancer Institute.
Application of Cy5B-trisulfo, NHS in microtubules.
Single-molecule localization microscopy (SMLM) images obtained using TIRF microscopy of microtubules immunolabeled with Cy5B-trisulfo, NHS labeled anti-tubulin antibody in 0.1% NaBH4/PBS upon irradiation with 640 nm (2.5 kWcm-2 ) using an integration time of 20 ms (total acquisition time 10 min). Scale bars, 3 μm and 1 μm (insets). Fourier ring correlation (FRC) analysis was used to estimate the indicated spatial resolutions. Image kindly provided by Dr. Martin Schnermann, Center for Cancer Research, National Cancer Institute.Licensing Information
Sold under license from the National Institute of HealthOptical Data
| Emission Color | Far Red |
| λabs | 669 nm |
| λem | 685 nm |
| Extinction Coefficient (ε) | 241,000 M-1cm-1 |
| Quantum Yield (φ) | 0.4 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Application | SMLM, FRET, smFRET, FLIM, dSTORM |
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Product Specifications for Cy5B-trisulfo, NHS
Molecular Weight
Formula
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InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Wäldchen Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM. Nat.Commun. 2020 PMID: 32060305
- Eiring Targetable conformationally restricted Cyanines enable photon-count-limited applications. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34606673
Product Documents for Cy5B-trisulfo, NHS
Certificate of Analysis
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Product Specific Notices for Cy5B-trisulfo, NHS
For research use only
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