Cyanine 5 Tyramide

Tocris Bioscience | Catalog # 6458

Red fluorescent reagent widely used for signal amplification in IHC and FISH
Tocris Bioscience

Key Product Details

Description

Red fluorescent reagent widely used for signal amplification in IHC and FISH

Alternative Names

Cy5 Tyramide

Wavelength

Excitation = 651 nm, Emission = 665 nm
View all Tyramide Signaling Amplification/TSA »

Product Description

Cyanine 5 Tyramide is a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data

Cyanine 5 Tyramide Dye Spectra
Emission Color Red
λabs 651 nm
λem 665 nm
Extinction Coefficient (ε) 250000 M-1cm-1
Closest Laser line 647 nm
Application Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Spectra Viewer

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Product Specifications for Cyanine 5 Tyramide

Molecular Weight

775.97

Formula

C41H49N3O8S2

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[5-[1,3-Dihydro-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino]-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt

CAS Number

1431148-26-3

PubChem ID

135290897

InChI Key

NHSJCIBSNKLAMA-UHFFFAOYSA-N

SMILES

CC[N+]1=C(C(C)(C2=CC(S([O-])(=O)=O)=CC=C12)C)/C=C/C=C/C=C3N(C4=CC=C(S(O)(=O)=O)C=C4C/3(C)C)CCCCCC(NCCC5=CC=C(C=C5)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.64 15

Preparing Stock Solutions for Cyanine 5 Tyramide

The following data is based on the product molecular weight 775.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.15 mM 8.59 mL 42.96 mL 85.91 mL
0.75 mM 1.72 mL 8.59 mL 17.18 mL
1.5 mM 0.86 mL 4.30 mL 8.59 mL
7.5 mM 0.17 mL 0.86 mL 1.72 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Cyanine 5 Tyramide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cyanine 5 Tyramide

For research use only

Citations for Cyanine 5 Tyramide

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