Recombinant Human Cyclophilin B Protein

Novus Biologicals | Catalog # NBC1-18424

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE
View all Cyclophilin B Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 26- 216 of Human Cyclophilin B

Source: E.coli

Amino Acid Sequence: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cyclophilin B Protein

SDS-PAGE: Recombinant Human Cyclophilin B Protein [NBC1-18424]

SDS-PAGE: Recombinant Human Cyclophilin B Protein [NBC1-18424]

SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]

Formulation, Preparation, and Storage

NBC1-18424
Formulation 20mM Tris-HCl buffer (pH 8.0), 20mM NaCl, 0.5mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin B

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Gene Symbol

PPIB

Additional Cyclophilin B Products

Product Documents for Recombinant Human Cyclophilin B Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cyclophilin B Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human Cyclophilin B Protein

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