Human Cytokeratin 1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-43175

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (100 uL)

Sensitivity

0.055 ng/mL (example only; lot dependent)

Assay Range

0.156-10 ng/mL (example only; lot dependent)
View all Cytokeratin 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Human Cytokeratin 1 ELISA Kit (Colorimetric)

Recovery

90 - 112% (example only; lot dependent)

Kit Contents for Human Cytokeratin 1 ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cytokeratin 1

Belonging to the keratin family, Cytokeratin 1 plays an important role in cell binding. More specifically, this gene is expressed in the spinous and granular layers of the epidermis with keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. This gene also regulates the activity of kinases such as PKC and SRC by binding to ITGB1 and the receptor of GNB2L1. Other biological processes include complement activation, lectin pathway, epidermis development, fibrinolysis, regulation of angiogenesis and response to oxidative stress.

Alternate Names

CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1

Gene Symbol

KRT1

Additional Cytokeratin 1 Products

Product Documents for Human Cytokeratin 1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Cytokeratin 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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