Cytokeratin 18 Antibody (C-04) [FITC]

Novus Biologicals | Catalog # NBP1-48350

Novus Biologicals
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Key Product Details

Species Reactivity

Mammal

Applications

Flow Cytometry, Flow (Intracellular)

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # C-04
View all Cytokeratin 18 Primary Antibodies »

Product Specifications

Immunogen

Cytoskeleton preparation of epidermal carcinoma cell line A431.

Marker

Epithelial Marker

Specificity

The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 18 Antibody (C-04) [FITC]

Cytokeratin 18 Antibody (C-04) [FITC]

Flow Cytometry: Mouse Monoclonal Cytokeratin 18 Antibody (C-04) [FITC] (NBP1-48350) -

Flow Cytometry: Mouse Monoclonal Cytokeratin 18 Antibody (C-04) [FITC] (NBP1-48350) - Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-Cytokeratin 18 (C-04) FITC antibody (concentration in sample 1.67 μg/ml).
Cytokeratin 18 Antibody (C-04) [FITC]

Flow Cytometry: Mouse Monoclonal Cytokeratin 18 Antibody (C-04) [FITC] (NBP1-48350) -

Flow Cytometry: Mouse Monoclonal Cytokeratin 18 Antibody (C-04) [FITC] (NBP1-48350) - Separation of HeLa cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with HeLa cells stained using anti-Cytokeratin 18 (C-04) FITC antibody (concentration in sample 1.67 μg/ml).

Applications for Cytokeratin 18 Antibody (C-04) [FITC]

Application
Recommended Usage

Flow (Intracellular)

1-2 ug/ml

Flow Cytometry

1-2 ug/ml
Application Notes
Western blot, IHC and Flow Cytometry. IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 18

Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3).

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (C-04) [FITC]

Certificate of Analysis

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Product Specific Notices for Cytokeratin 18 Antibody (C-04) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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