D-Luciferin sodium salt

Tocris Bioscience | Catalog # 5427

Firefly luciferase substrate; cell permeable.
Tocris Bioscience

Key Product Details

Description

Firefly luciferase substrate; cell permeable.
View all Bioluminescent Substrates »

Product Description

D-Luciferin sodium salt is a firefly luciferase substrate (Km = ~2 μM). Used in many standard bioluminescence imaging (BLI) techniques, including monitoring tumour growth, and investigating intracellular signaling activity in vitro and in vivo. Used with MC3 LNP Luciferase (Cat. No. 8889) for in vitro and in vivo bioluminescence LNP detection.Cell permeable. Emission maximum = 562 nm.

Optical Data

D-Luciferin sodium salt Dye Spectra
Emission Color Green
λem 562 nm
Cell Permeable Yes
Application Bioluminescence Imaging (BLI) Techniques, including monitoring tumor growth

Spectra Viewer

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Product Specifications for D-Luciferin sodium salt

Molecular Weight

302.30

Formula

C11H7N2NaO3S2

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt

CAS Number

103404-75-7

PubChem ID

23710675

InChI Key

BZNVUYVALNTPBG-SREJTOIWSA-M

SMILES

OC1=CC2=C(N=C(C3=N[C@@H]([C@@]([O-])=O)CS3)S2)C=C1.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 30.23 100
DMSO 30.23 100

Preparing Stock Solutions for D-Luciferin sodium salt

The following data is based on the product molecular weight 302.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.31 mL 16.54 mL 33.08 mL
5 mM 0.66 mL 3.31 mL 6.62 mL
10 mM 0.33 mL 1.65 mL 3.31 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 9 publications citing the usage of this product.

Product Documents for D-Luciferin sodium salt

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for D-Luciferin sodium salt

For research use only

Citations for D-Luciferin sodium salt

Customer Reviews for D-Luciferin sodium salt (1)

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  • D-Luciferin was used to assess ATP availability in cellular assays
    Name: Sam Sreshtha
    Species: Human
    Verified Customer | Posted 08/18/2019
    D-Luciferin was used to assess ATP availability in cellular assays.The fluorescent images were distinguishable and publish worthy.
    D-Luciferin sodium salt 5427

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