DA ZP1

Tocris Bioscience | Catalog # 7444

Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells
Tocris Bioscience

Key Product Details

Description

Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells

Wavelength

Excitation = 490 nm, Emission = 522 nm
View all Fluorescent Cell Indicators and Sensors »

Product Description

DA ZP1 (diacetylated Zinpyr1) is a fluorogenic Zn(II) sensor (Kd Zn2+ = 0.6 nM). It can be used to image and isolate pancreatic β-cells and β-like cells in vitro. Used to purify live stem cell-derived β-like cells. It can image transplanted islet grafts and endogenous mouse islets in vivo. DA ZP1 is non-fluorescent in the absence of Zn(II) ions but its binding with Zn(II) promotes hydrolytic cleavage of the acetyl groups, generating a strong fluorescent signal. Excitation and emission maxima (λ) are 490 nm and 522 nm, respectively.

Optical Data

λabs 490 nm
λem 522 nm
Cell Permeable Yes
Application Flow Cytometry

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Product Specifications for DA ZP1

Molecular Weight

907.81

Formula

C50H40Cl2N6O7

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

3',6'-bis(Acetyloxy)-4',5'-bis[[bis(2-pyridinylmethyl)amino]methyl]-2',7'-dichlorospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

CAS Number

1816254-69-9

PubChem ID

86305621

InChI Key

HPUUCJUOCSGDIK-UHFFFAOYSA-N

SMILES

CC(OC1=C(C=C2C(OC3=C(C(OC(C)=O)=C(C=C3C24OC(C5=CC=CC=C45)=O)Cl)CN(CC6=CC=CC=N6)CC7=CC=CC=N7)=C1CN(CC8=CC=CC=N8)CC9=CC=CC=N9)Cl)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.82 2

Preparing Stock Solutions for DA ZP1

The following data is based on the product molecular weight 907.81.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.02 mM 55.08 mL 275.39 mL 550.78 mL
0.1 mM 11.02 mL 55.08 mL 110.16 mL
0.2 mM 5.51 mL 27.54 mL 55.08 mL
1 mM 1.10 mL 5.51 mL 11.02 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for DA ZP1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DA ZP1

For research use only

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