DAF FM diacetate

Tocris Bioscience | Catalog # 7756

Cell permeable photostable nitric oxide (NO) fluorescent indicator
Tocris Bioscience

Key Product Details

Description

Cell permeable photostable nitric oxide (NO) fluorescent indicator

Wavelength

Excitation = 495 nm, Emission = 515 nm
View all Fluorescent Cell Indicators and Sensors »

Product Description

DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).

It is recommended to prepare stock solutions in DMSO.

Optical Data

DAF FM diacetate Dye Spectra
Emission Color Green
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 84000 M-1cm-1
Cell Permeable Yes
pH Sensitivity Independent of pH when pH >5.8
Application Confocal Microscopy, Flow cytometry

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Product Specifications for DAF FM diacetate

Molecular Weight

496.42

Formula

C25H18F2N2O7

Storage

Store at -20°C

Purity

≥80% (HPLC)

Chemical Name

4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate

CAS Number

254109-22-3

PubChem ID

2762646

InChI Key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

SMILES

O=C1OC2(C3=CC=C(C(N)=C13)NC)C4=CC(F)=C(C=C4OC5=CC(OC(C)=O)=C(C=C52)F)OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Product Documents for DAF FM diacetate

Certificate of Analysis

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Product Specific Notices for DAF FM diacetate

For research use only

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