DAF FM diacetate New
Chemical Name: 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate
Biological ActivityDAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to a cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).
It is recommended to prepare stock solutions in DMSO.
Optical Data for DAF FM diacetate
|Extinction Coefficient (ε)||84000 M-1cm-1|
|pH Sensitivity||Independent of pH when pH >5.8|
|Application||Confocal imaging, Flow cytometry|
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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