DBF4B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13900

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DBF4B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: HPSQENSFAPADIPVKGPLLFPEARPWLMSARCWVRPFPFVTWGCLIPHDTTPLHEEVSPCPCLRLGYLYLLLTQSLWCRVRVPSLSTAGPIPRTSHPCTLAFPSYLNDHDLGHLCQAKPQGWNTPQPFLHCGFLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DBF4B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DBF4B Antibody [NBP2-13900]

Immunocytochemistry/ Immunofluorescence: DBF4B Antibody [NBP2-13900]

Immunocytochemistry/Immunofluorescence: DBF4B Antibody [NBP2-13900] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: DBF4B Antibody [NBP2-13900]

Immunohistochemistry-Paraffin: DBF4B Antibody [NBP2-13900]

Immunohistochemistry-Paraffin: DBF4B Antibody [NBP2-13900] - Staining of human rectum shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Applications for DBF4B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DBF4B

DBF4B, also known as Protein DBF4 homolog B, consists of four isoforms of sizes 67.2 kDa, 47.2 kDa, 54.7 kDa, and 18.4 kDa and is involved in DNA replication and cellular generation by stimulating kinase activity. The protein has not been researched in association with any diseases. The protein interacts with PTEN, APC, CDC7, MDFI, and KRTAP4-12 proteins during the early S phase of cell cycle DNA replication.

Alternate Names

Activator of S phase kinase-like protein 1, activator of S-phase kinase-like protein 1, ASKL1CHIFB, ASK-like protein 1, chifb, Chiffon homolog B, DBF4 homolog B (S. cerevisiae), Dbf4-related factor 1, DRF1MGC15009, FLJ13087, protein DBF4 homolog B, ZDBF1B, zinc finger, DBF-type containing 1B

Gene Symbol

DBF4B

Additional DBF4B Products

Product Documents for DBF4B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DBF4B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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