DCST1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-59775
![Western Blot: DCST1 Antibody [NBP1-59775]](https://resources.rndsystems.com/images/products/DCST1-Antibody-Western-Blot-NBP1-59775-img0001.jpg "Western Blot: DCST1 Antibody [NBP1-59775]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptides corresponding to DCST1(DC-STAMP domain containing 1) The peptide sequence was selected from the C terminal of DCST1. Peptide sequence SYVCRTLDCEAVYCWSCWDDMRQRCPVCTPREELSSSAFSDSNDDTAYAG. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for DCST1 Antibody - BSA Free
Western Blot: DCST1 Antibody [NBP1-59775]
Western Blot: DCST1 Antibody [NBP1-59775] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml![Immunohistochemistry-Paraffin: DCST1 Antibody [NBP1-59775]](https://resources.rndsystems.com/images/products/DCST1-Antibody-Immunohistochemistry-Paraffin-NBP1-59775-img0002.jpg "Immunohistochemistry-Paraffin: DCST1 Antibody [NBP1-59775]")
Immunohistochemistry-Paraffin: DCST1 Antibody [NBP1-59775]
Immunohistochemistry-Paraffin: DCST1 Antibody [NBP1-59775] - Human Heart Tissue, antibody concentration 5.0ug/ml.Applications for DCST1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DCST1
Alternate Names
DC-STAMP domain containing 1, DC-STAMP domain-containing protein 1, FLJ32785, RP11-307C12.10
Gene Symbol
DCST1
UniProt
Additional DCST1 Products
Product Documents for DCST1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DCST1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for DCST1 Antibody - BSA Free
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Q: Can we apply DCST1 Antibody (NBP1-59775) to human breast cancer tissue paraffin?
A: Our DCST1 Antibody (NBP1-59775) antibody has been validated for IHC-P in Human Heart Tissue, so this application/species is covered by our guarantee. We do have an IHC-P image on the product's datasheet. However, we have not tested this antibody specifically with breast cancer tissue yet. Technically, if the breast tissues that you are planning to test is expressing DCST1 (at a significant level), then yes, the antibody should be suitable for your experiments.
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