DFHBI 1T

Catalog # Availability Size / Price Qty
5610/10
DFHBI 1T | CAS No. 1539318-36-9 | Light-up Aptamers
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Description: GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers

Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one

Purity: ≥98%

Product Details
Citations (1)
Reviews

Biological Activity

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (Kd = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Technical Data

M.Wt:
320.21
Formula:
C13H9F5N2O2
Solubility:
Soluble to 100 mM in DMSO and to 50 mM in ethanol
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
1539318-36-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citation for DFHBI 1T

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