Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Biological ActivityMimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Plug-and-play fluorophores extend the spectral properties of Spinach.
Song et al.
In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.
Filonov et al.
Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes.
Svensen and Jaffrey
Cell Chem.Biol., 2016;23:415
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