Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Biological ActivityDFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (Kd = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.
Filonov et al.
Plug-and-play fluorophores extend the spectral properties of Spinach.
Song et al.
Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes.
Svensen and Jaffrey
Cell Chem.Biol., 2016;23:415
Citations for DFHBI 1T
The citations listed below are publications that use Tocris products. Selected citations for DFHBI 1T include:
4 Citations: Showing 1 - 4
Quantitative RNA imaging in single live cells reveals age-dependent asymmetric inheritance.
Authors: Robert Et al.
Cell Rep 2022;41:111656
Specific Nucleic AcId Ligation for the detection of Schistosomes: SNAILS.
Authors: Paul S Et al.
PLoS Negl Trop Dis 2022;16:e0010632
Cell-free biosensors for rapid detection of water contaminants.
Authors: Jeong Wook Et al.
Nat Biotechnol 2020;38:1451-1459
Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems.
Authors: Yim Et al.
Mol Syst Biol 2019;15:e8875
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