DFHBI 1T

Tocris Bioscience | Catalog # 5610

GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Tocris Bioscience

Key Product Details

Description

GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
View all Light-up Aptamers »

Product Description

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (Kd = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

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Product Specifications for DFHBI 1T

Molecular Weight

320.21

Formula

C13H9F5N2O2

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one

CAS Number

1539318-36-9

PubChem ID

101889712

InChI Key

AWYCLBWNRONMQC-WMZJFQQLSA-N

SMILES

O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.02 100

Preparing Stock Solutions for DFHBI 1T

The following data is based on the product molecular weight 320.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.23 mL
5 mM 0.62 mL 3.12 mL 6.25 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 30 publications citing the usage of this product.

Product Documents for DFHBI 1T

Certificate of Analysis

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Product Specific Notices for DFHBI 1T

For research use only

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