Catalog Number: 5609
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
Biological Activity
Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Plug-and-play fluorophores extend the spectral properties of Spinach.
    Song et al.
    J.Am.Chem.Soc., 2014;136:198
  2. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.
    Filonov et al.
    Chem.Biol., 2015;22:649
  3. RNA mimics of green fluorescent protein.
    Paige et al.
    Science, 2011;333:642
  4. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria.
    Strack et al.
    Nat.Protoc., 2014;9:146
  5. Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes.
    Svensen and Jaffrey
    Cell Chem.Biol., 2016;23:415
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