DFHBI (CAS 1241390-29-3): R&D Systems
Catalog Number: 5609
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
Biological Activity
Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.
Technical Data
  • M.Wt:
    252.22
  • Formula:
    C12H10F2N2O2
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Store at -20°C
  • CAS No:
    1241390-29-3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Plug-and-play fluorophores extend the spectral properties of Spinach.
    Song et al.
    J.Am.Chem.Soc., 2014;136:198
  2. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.
    Filonov et al.
    Chem.Biol., 2015;22:649
  3. RNA mimics of green fluorescent protein.
    Paige et al.
    Science, 2011;333:642
  4. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria.
    Strack et al.
    Nat.Protoc., 2014;9:146
  5. Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes.
    Svensen and Jaffrey
    Cell Chem.Biol., 2016;23:415

Have you used DFHBI?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

Order Details

Note:

Contact Information

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

R&D Systems Guarantee

Feedback

Customer Information