DHEA ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-07909

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Culture supernatants, plasma, serum, saliva, and urine (100 ul)

Sensitivity

2.9 pg/ml

Assay Range

12.21 - 50000 pg/ml
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

Cross reactivities for a number of related steroid compounds:

DHEA sulfate 30%
Androstenedione 0.72%
Androsterone 0.29%
Pregnenolone 0.28%
Testosterone 0.10%
Progesterone 0.06%
Estradiol 0.04%
20alpha-hydroxycholesterol 0.02%
Androsterone sulphate 0.02%
DHEA sodium sulfate 0.02%
17alpha-hydroxyepipregnanolone 0.02%
19-hydroxyandrostendione 0.02%
17alpha-hydroxyprogesterone 0.02%
Cortisol 0.02%

Precision

Intra-Assay Precision (Precision within an assay) CV% 10%

Inter-Assay Precision (Precision between assays) CV% 10%

Recovery for DHEA ELISA Kit (Colorimetric)

Recovery

Tissue Culture Media: 95.2%
Human Saliva: 109.8%
Human Urine: 108.4%
Human Serum: 98.5%
Human heparin Plasma: 100.4%

Scientific Data Images for DHEA ELISA Kit (Colorimetric)

Non-species specific DHEA ELISA Kit (Colorimetric) [NBP3-07909] - Standard curve

Kit Contents for DHEA ELISA Kit (Colorimetric)

  • Assay Buffer Concentrate
  • DHEA Assay Layout Sheet
  • DHEA ELISA Antibody
  • DHEA ELISA Conjugate
  • DHEA Standard
  • Goat anti-Rabbit IgG Plates
  • Plate Sealer
  • pNpp Substrate
  • Stop Solution
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Product Documents for DHEA ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for DHEA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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