Catalog # Availability Size / Price Qty
Di 4 ANEPPS | CAS No. 90134-00-2 | Fluorescent Cell Indicators and Sensors
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Description: Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols

Chemical Name: 6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate

Purity: ≥90%

Product Details

Biological Activity

Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.

In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Di 4 ANEPPS

λabs 465 nm
λem 635 nm
Application Electrophysiology, Fluorescence Microscopy

Technical Data

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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