Di 4 ANEPPS

Tocris Bioscience | Catalog # 7324

Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
Tocris Bioscience

Key Product Details

Description

Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols

Wavelength

Excitation = 465 nm, Emission = 635 nm
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Product Description

Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.

In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.

It is recommended to prepare stock solutions in DMSO.

Optical Data

Emission Color Red
λabs 465 nm
λem 635 nm
Application Electrophysiology, Fluorescence Microscopy

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Product Specifications for Di 4 ANEPPS

Molecular Weight

480.67

Formula

C28H36N2O3S

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate

CAS Number

90134-00-2

PubChem ID

6438327

InChI Key

HAPJROQJVSPKCJ-UHFFFAOYSA-N

SMILES

[O-]S(CCC[N+](C=C1)=CC=C1/C=C/C(C=C2C=C3)=CC=C2C=C3N(CCCC)CCCC)(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Product Documents for Di 4 ANEPPS

Certificate of Analysis

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Product Specific Notices for Di 4 ANEPPS

For research use only

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