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CellSperse

Catalog #: 3450-048-05
2 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

3450-048-05 has been discontinued.
View all Angiogenesis Assay Kits products.
Product Details
Citations (2)
FAQs
Reviews (1)
Summary Product Datasheets

CellSperse Summary

DIVAA CellSperse is provided as an enzymatic matrix and cell dissociation reagent for the Directed In Vivo Angiogenesis Assay (DIVAA).

Why Use CellSperse?

DIVAA CellSperse is a neutral metalloprotease from Bacillus polymyxa that promotes BME digestion and gentle cell dissociation for angiogenesis quantification in the Directed In Vivo Angiogenesis Assay (DIVAA).

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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Product Datasheet
COA
SDS

Citations for CellSperse

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Extracellular Matrix Collagen I Differentially Regulates the Metabolic Plasticity of Pancreatic Ductal Adenocarcinoma Parenchymal Cell and Cancer Stem Cell
    Authors: Tavares-Valente, D;Cannone, S;Greco, MR;Carvalho, TMA;Baltazar, F;Queirós, O;Agrimi, G;Reshkin, SJ;Cardone, RA;
    Cancers  2023-07-29
  2. Extracellular Matrix composition modulates PDAC parenchymal and stem cell plasticity and behavior through the secretome
    Authors: G Biondani, K Zeeberg, MR Greco, S Cannone, I Dando, ED Pozza, M Mastrodona, S Forciniti, V Casavola, M Palmieri, SJ Reshkin, RA Cardone
    FEBS J., 2018-04-27;0(0):.  2018-04-27

FAQs

  1. Can cells grown in Basement Membrane Extract (BME), such as Catalog # 3433-005-01, be used in Flow Cytometry? 

    • For preparation of a single cell suspension for applications such as FACS and re-culturing, digestion of the matrix and cell-cell bonds may be facilitated using enzymes such as Trypsin or Dispase. Our CellSperse, cat# 3450-048-05, is similar to Dispase, and can also be used for this purpose. However, some markers may be susceptible to degradation from CellSperse treatment. It is recommended to consult the literature for design of experiments for the particular cell type and markers of interest.

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Reviews for CellSperse

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DIVAA Cell Sperse
By Leslie Priddy on 04/01/2018