DLDVPIPGRFDRRVS(PO3)VAAE Ser/Thr Phosphatase Substrate I

R&D Systems | Catalog # ES012

Substrate for Calcineurin, PP1, PP2A, PP2C, and Ser/Thr phosphatases
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Product Specifications

Source


Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO3)-Val-Ala-Ala-Glu
Synthetic phosphopeptide corresponding to amino acids 81 to 99 of Protein Kinase A Regulatory Subunit 2-alpha (RII, Accession # P00515).

Predicted Molecular Mass

2192 Da

Formulation, Preparation, and Storage

ES012
Formulation Lyophilized from a stock solution in distilled water.
Reconstitution Dissolves in water.
36.4 μL gives a stock solution of substrate at a 10 mM concentration.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Lyophilized samples are stable for up to twelve months from date of receipt at -20° C to -70° C. Upon reconstitution, the substrate can be stored at 2° - 8° C for one month or at -20° to -70° C in a manual defrost freezer for three months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phosphatase Peptide Substrates

This phosphopeptide is a substrate for a variety of serine/threonine protein phosphatases (PPs), including PP1, PP2A, PP2B (calcineurin) and PP2C. Enzymatic cleavage of this substrate liberates inorganic phosphate, which can be measured in dye binding assays, such as the malachite green method.

References

  1. Pinna, L.A. and A. Donella-Deana (1994) Biochim. Biophys. Acta 1222:415.
  2. Donella-Deana, A. et al. (1994) Eur. J. Biochem. 219:109.
  3. McNall, S.J. and E.H. Fischer (1988) J. Biol. Chem. 263:1893.
  4. Fathi, A-R. et al. (2002) Anal. Biochem. 310:208.
  5. Van Veldhoven, P.P. and G.P. Mannaerts (1987) Anal. Biochem. 161:45.

Additional Phosphatase Peptide Substrates Products

Product Documents for DLDVPIPGRFDRRVS(PO3)VAAE Ser/Thr Phosphatase Substrate I

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Product Specific Notices for DLDVPIPGRFDRRVS(PO3)VAAE Ser/Thr Phosphatase Substrate I

For research use only

Citations for DLDVPIPGRFDRRVS(PO3)VAAE Ser/Thr Phosphatase Substrate I

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