DMSO, sterile filtered
Tocris Bioscience | Catalog # 3176
Key Product Details
Description
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Product Description
DMSO, sterile filtered is a solvent with wide ranging applications in cell culture, cryopreservation and organic chemistry. DMSO dissolves both polar and nonpolar compounds. Improves responsiveness of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to differentiation signals. DMSO is used in PCR to inhibit secondary structure formation by DNA template or primers.
DMSO, Cell Cryopreserve Grade (Cat. No. 7726) also available.
Product Specifications for DMSO, sterile filtered
Molecular Weight
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The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 40 publications citing the usage of this product.
- Chetty A simple tool to improve pluripotent stem cell differentiation. Nat.Methods 2013 PMID: 23584186
Product Documents for DMSO, sterile filtered
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DMSO, sterile filtered
For research use only
Citations for DMSO, sterile filtered
Customer Reviews for DMSO, sterile filtered (6)
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Customer Images
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Species: HamsterAssay Type: In VitroCell Line/Tissue: Baby Hamster Kidney fibroblasts (BHK cells)Verified Customer | Posted 08/18/2025Cryopreservation solution: 10% DMSO + 90% FBS.I used DMSO for cryopreservation of BHK cells. The cells recover well after thaw.
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Species: HumanAssay Type: In VitroVerified Customer | Posted 07/22/2025The DMSO was used to resuspend a lyophilised viability dye for flow cytometry experiments, as well as various proliferation inhibitors for in vitro assays.
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Species: HumanAssay Type: In VitroVerified Customer | Posted 07/11/2025Vehicle and solvent for small molecules.
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Species: MouseAssay Type: In VivoCell Line/Tissue: colorectal cancer cellsVerified Customer | Posted 08/14/2023I used this DMSO for in vivo mice injections. The product worked very well in vivo. 10% DMSO solution was prepared in sterile PBS and the mice were injected with 200-300 uL i.p. every other day. All mice tolerated the injection and survived very well for more than 4 weeks.
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Species: HumanAssay Type: In VitroCell Line/Tissue: HBEC-5i (ATCC CRL-3245)Verified Customer | Posted 01/25/2021We use DMSO for cryopreservation of HBECs - 92.5% of complete growth medium (containing DMEM/F12, 10% FBS, 1% penicillin-streptomycin and 40 µg/mL endothelial growth supplement) : 7.5% of DMSO. Cells grow well after thawing.
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Species: HumanAssay Type: In VitroCell Line/Tissue: iPSCVerified Customer | Posted 08/07/2018Make 100µl aliquots and keep them at −80 °C for 6-12 months.DMSO is used for dissolving CHIR (4423). Reconstitute 10 mg of CHIR in 2.149 ml of DMSO for a final concentration of 10mM.
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FAQs for DMSO, sterile filtered
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Q: What type of vial is Catalog # 3176, DMSO, supplied in?
A: The 5 mL and 25 mL size products are supplied in 5 mL glass vials. The 100 mL product is supplied in a 100 mL glass vial.