DNA Polymerase delta, catalytic subunit Antibody (6196) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43556

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6196

Format

Azide and BSA Free
View all DNA Polymerase delta, catalytic subunit Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

DNA Polymerase delta, catalytic subunit Antibody (6196) - Azide and BSA Free

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (6196) [NBP2-43556] -

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody [6196] (NBP2-43556) diluted at 1:1000.
DNA Polymerase delta, catalytic subunit Antibody (6196) - Azide and BSA Free

Immunohistochemistry-Paraffin: DNA Polymerase delta, catalytic subunit Antibody (6196) [NBP2-43556] -

DNA polymerase delta antibody [6196] detects DNA polymerase delta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
DNA polymerase delta stained by DNA polymerase delta antibody [6196] (NBP2-43556) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Polymerase delta, catalytic subunit

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Alternate Names

CDC2, CDC2 homolog, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, DNA-directed polymerase delta 1, EC 2.7.7.7, POLD, polymerase (DNA directed), delta 1, catalytic subunit (125kD), polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Gene Symbol

POLD1

Additional DNA Polymerase delta, catalytic subunit Products

Product Documents

Certificate of Analysis

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Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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