DNA Polymerase iota Antibody (OTI3B5)

Novus Biologicals | Catalog # NBP3-44668

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B5
View all DNA Polymerase iota Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 458-740 of human POLI (NP_009126) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for DNA Polymerase iota Antibody (OTI3B5)

DNA Polymerase iota Antibody (OTI3B5) Western Blot;DNA Polymerase iota Antibody (OTI3B5)[NBP3-44668]-

Western Blot;DNA Polymerase iota Antibody (OTI3B5)[NBP3-44668]-

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-POLI monoclonal antibody (1:200).
DNA Polymerase iota Antibody (OTI3B5) Immunohistochemistry;DNA Polymerase iota Antibody (OTI3B5)[NBP3-44668]-

Immunohistochemistry;DNA Polymerase iota Antibody (OTI3B5)[NBP3-44668]-

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLI mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
DNA Polymerase iota Antibody (OTI3B5) Western Blot;DNA Polymerase iota Antibody (OTI3B5)[NBP3-44668]-

Western Blot;DNA Polymerase iota Antibody (OTI3B5)[NBP3-44668]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY POLI ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI. Positive lysates

Applications for DNA Polymerase iota Antibody (OTI3B5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNA Polymerase iota

DNA polymerase Iota (POLI), also known as Rad30B is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in DNA Pol I.

DNA Pol I antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Alternate Names

DNA polymerase iota, EC 2.7.7.7, eta2, polymerase (DNA directed) iota, RAD30 homolog B, RAD30Bpolymerase (DNA-directed), iota, RAD3OB

Gene Symbol

POLI

Additional DNA Polymerase iota Products

Product Documents for DNA Polymerase iota Antibody (OTI3B5)

Certificate of Analysis

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Product Specific Notices for DNA Polymerase iota Antibody (OTI3B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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