DOT1L Antibody - BSA Free

Novus Biologicals | Catalog # NB100-40846

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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DOT1L Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue1485 and the C-terminus (residue 1537) of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOT1L Antibody - BSA Free

Immunoprecipitation: DOT1L Antibody [NB100-40846]

Immunoprecipitation: DOT1L Antibody [NB100-40846]

Immunoprecipitation: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds.

Applications for DOT1L Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Entrez Gene IDs

84444 (Human)

Gene Symbol

DOT1L

UniProt

NP_115871.1 (Human)

Additional DOT1L Products

Product Documents for DOT1L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DOT1L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for DOT1L Antibody - BSA Free

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Protocols

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FAQs

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