Doxycycline hyclate

Tocris Bioscience | Catalog # 4090

Broad-spectrum MMP inhibitor; tetracycline derivative
Tocris Bioscience

Key Product Details

Description

Broad-spectrum MMP inhibitor; tetracycline derivative
View all Synthetic Metalloprotease Inhibitors »

Product Description

Doxycycline hyclate is a broad-spectrum antibiotic and MMP inhibitor; tetracycline derivative. Doxycycline hyclate increases and promotes smooth muscle cell adhesion; inhibits proliferation and migration. Protects the microvasculature by inhibiting plasminogen systems. Doxycycline hyclate is also used as the regulator for inducible gene expression systems which depends on either the presence (TeT-On) or absence (TeT-Off) of Doxycycline. Used in protocols for reprogramming MEFs into skeletal muscle progenitors (see our protocol below).

For more information about how Doxycycline hyclate may be used, see our protocols: Reprogramming MEFs into Skeletal Muscle Progenitors.

Product Specifications for Doxycycline hyclate

Molecular Weight

512.94

Formula

C22H24N2O8.HCl.0.5C2H6O.0.5H2O

Storage

Store at +4°C

Purity

≥92% (HPLC)

Chemical Name

(4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)- 3,5,10,12,12a-pentahydroxy- 6-methyl- 1,11-dioxo- 1,4,4a,5,5a,6,11,12a-octahydrotetracene- 2-carboxamide hydrochloride hemiethanolate hemihydrate

CAS Number

24390-14-5

PubChem ID

54686183

InChI Key

UHHHTIKWXBRCLT-VDBOFHIQSA-N

SMILES

OC1=C(C(C2=C(O)[C@@](C(C(C(N)=O)=C(O)[C@H]4N(C)C)=O)(O)[C@@]4([H])[C@@H](O)[C@@]([H])2[C@H]3C)=O)C3=CC=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 48.09 100
DMSO 48.09 100

Preparing Stock Solutions for Doxycycline hyclate

The following data is based on the product molecular weight 512.94.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.95 mL 9.75 mL 19.50 mL
5 mM 0.39 mL 1.95 mL 3.90 mL
10 mM 0.19 mL 0.97 mL 1.95 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 23 publications citing the usage of this product.

Product Documents for Doxycycline hyclate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Doxycycline hyclate

For research use only

⚠ WARNING: This product can expose you to chemicals including Doxycycline hyclate (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for Doxycycline hyclate

Customer Reviews for Doxycycline hyclate (1)

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  • Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/09/2019
    Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa. E coli was used as a standard to compare.
    Doxycycline hyclate 4090

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