DPH2 Antibody (OTI4E7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70585

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E7

Format

Azide and BSA Free
View all DPH2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DPH2 Antibody (OTI4E7) - Azide and BSA Free

Western Blot: DPH2 Antibody (OTI4E7)Azide and BSA Free [NBP2-70585]

Western Blot: DPH2 Antibody (OTI4E7)Azide and BSA Free [NBP2-70585]

Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human prostate tissue using anti-DPH2 mouse monoclonal antibody.
Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DPH2 antibody, and then analyzed by flow cytometry.
Western Blot: DPH2 Antibody (OTI4E7)Azide and BSA Free [NBP2-70585]

Western Blot: DPH2 Antibody (OTI4E7)Azide and BSA Free [NBP2-70585]

Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DPH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human colon tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human endometrium tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human liver tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human pancreas tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human tonsil using anti-DPH2 mouse monoclonal antibody.

Applications for DPH2 Antibody (OTI4E7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DPH2

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

diphthamide biosynthesis protein 2, Diphthamide biosynthesis protein 2 homolog-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae), DPH2 homolog, DPH2 homolog (S. cerevisiae), DPH2L2diphthamide biosynthesis-like protein 2, DPH2-like 2, DPH2-like 2 (S. cerevisiae), HsDph2

Gene Symbol

DPH2

Additional DPH2 Products

Product Documents for DPH2 Antibody (OTI4E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DPH2 Antibody (OTI4E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DPH2 Antibody (OTI4E7) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review DPH2 Antibody (OTI4E7) - Azide and BSA Free and earn rewards!

Have you used DPH2 Antibody (OTI4E7) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies