dTAG Janelia Fluor® 525

Tocris Bioscience | Catalog # 8102

Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins
Tocris Bioscience

Key Product Details

Description

Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins

Wavelength

Excitation = 530 nm, Emission = 560 nm
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Product Description

Key Information: dTAG Janelia Fluor® 525 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.

Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 525 is cell permeable.

Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (73%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 525; excitation and emission maxima (λ) are 530 nm and 560 nm, respectively; quantum yield = 0.47; extinction coefficient = 82,800 M-1cm-1 (in 4% TFA).

It is recommended to use a working concentration of 10 μM in live cell imaging application.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Optical Data

Emission Color Yellow
Brightness
λabs 530 nm
λem 560 nm
Extinction Coefficient (ε) 82800 M-1cm-1
Quantum Yield (φ) 0.47
Closest Laser line 488 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy

Spectra Viewer

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Product Specifications for dTAG Janelia Fluor® 525

Molecular Weight

1244.30

Formula

C67H69F4N5O14

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)benzoate

InChI Key

ZSEJCFOGOGMEPJ-FUJQAITOSA-N

SMILES

O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)(F)C6)C=C5O7)=C(C=C/8)C7=CC8=[N+]9CC(F)(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12.44 10

Preparing Stock Solutions for dTAG Janelia Fluor® 525

The following data is based on the product molecular weight 1244.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 8.04 mL 40.18 mL 80.37 mL
0.5 mM 1.61 mL 8.04 mL 16.07 mL
1 mM 0.80 mL 4.02 mL 8.04 mL
5 mM 0.16 mL 0.80 mL 1.61 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for dTAG Janelia Fluor® 525

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dTAG Janelia Fluor® 525

For research use only

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