dTAG Janelia Fluor® 585

Tocris Bioscience | Catalog # 8103

Fluorogenic srTAG probe for live cell imaging of FKBP12(F36V/L) fusion proteins
Tocris Bioscience

Key Product Details

Description

Fluorogenic srTAG probe for live cell imaging of FKBP12(F36V/L) fusion proteins

Wavelength

Excitation = 590 nm, Emission = 620 nm
View all Fluorescent Probes »

Product Description

Key Information: dTAG Janelia Fluor® 585 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.

Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 585 is cell permeable.

Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (70%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 585; excitation and emission maxima (λ) are 590 nm and 620 nm, respectively; quantum yield = 0.52; extinction coefficient = 123,000 M-1cm-1 (in 4% TFA).

It is recommended to use a working concentration of 10 μM in live cell imaging application.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Optical Data

Emission Color Orange
Brightness
λabs 590 nm
λem 620 nm
Extinction Coefficient (ε) 123000 M-1cm-1
Quantum Yield (φ) 0.52
Closest Laser line 561 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy

Spectra Viewer

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Product Specifications for dTAG Janelia Fluor® 585

Molecular Weight

1270.39

Formula

C70H75F4N5O13

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-10,10-dimethyl-3,10-dihydroanthracen-9-yl)-4-((2-(2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)benzoate

InChI Key

TWOFEXPETOXCCT-XMAFRJPPSA-N

SMILES

O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)(F)C6)C=C5C7(C)C)=C(C=C/8)C7=CC8=[N+]9CC(F)(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12.7 10

Preparing Stock Solutions for dTAG Janelia Fluor® 585

The following data is based on the product molecular weight 1270.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 7.87 mL 39.36 mL 78.72 mL
0.5 mM 1.57 mL 7.87 mL 15.74 mL
1 mM 0.79 mL 3.94 mL 7.87 mL
5 mM 0.16 mL 0.79 mL 1.57 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for dTAG Janelia Fluor® 585

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dTAG Janelia Fluor® 585

For research use only

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