DuoSet ELISA Ancillary Reagent Kit 1

R&D Systems | Catalog # DY007

Product in process of being discontinued and replaced by new Next generation kit
R&D Systems
Discontinued Product
DY007 has been discontinued. An alternative/replacement product is available: DY007B. View all Buffers, Chelators and Reagents products.

Key Product Details

Applications

ELISA

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Product Summary for DuoSet ELISA Ancillary Reagent Kit 1

Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays
PRODUCT DETAIL

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 1 contains Reagent Diluent 1. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.

PRODUCT FEATURES
  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1.4% delipidized bovine serum, 0.05% Tween® 20 in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.
KIT CONTENTS
  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • 20X PBS
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 1 (one 21 mL vial of a buffered protein base)
  • Wash Buffer (25X, seven 21 mL vials)
  • Reagent Diluent Concentrate 3 (5X, two 21 mL vials of a 25% Tween 20 solution in PBS).
    For Use as the blocking buffer.

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for DuoSet ELISA Ancillary Reagent Kit 1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for DuoSet ELISA Ancillary Reagent Kit 1

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for DuoSet ELISA Ancillary Reagent Kit 1

Customer Reviews for DuoSet ELISA Ancillary Reagent Kit 1 (8)

4.9 out of 5
8 Customer Ratings
5 Stars
88%
4 Stars
13%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 8 reviews Showing All
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  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 10/05/2023
    Very nice dose-response curve using ancillary kit. The kit is very stable.
    DuoSet ELISA Ancillary Reagent Kit 1 DY007
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 01/19/2022
    works perfectly with il-1
    DuoSet ELISA Ancillary Reagent Kit 1 DY007
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 12/04/2020
    Used with the Mouse TGF-beta 1 DuoSet ELISA. Performed with precision from one plate to the next. Instructions were complete and easy to follow.
    DuoSet ELISA Ancillary Reagent Kit 1 DY007
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 10/29/2019
    Confusing reagent labels especially blocking buffer
    DuoSet ELISA Ancillary Reagent Kit 1 DY007
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 08/28/2019
    instructions kind of confusing - have the directions are on the ancillary kit and the other are with the antibody kit. Also some of the buffers names need to be clearer and more consistent (e.g. blocking and coating). Results are good though so thats what matters.
    DuoSet ELISA Ancillary Reagent Kit 1 DY007
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 04/05/2019
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 10/17/2018
  • DuoSet ELISA Ancillary Reagent Kit 1
    Name: Anonymous
    Verified Customer | Posted 09/18/2018

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