DuoSet ELISA Ancillary Reagent Kit 1

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R&D Systems DuoSet Ancillary Reagent Kits
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Product Details
Citations (2)
Reviews (6)

DuoSet ELISA Ancillary Reagent Kit 1 Summary

Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 1 contains Reagent Diluent 1. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.

  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1.4% delipidized bovine serum, 0.05% Tween® 20 in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • 20X PBS
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 1 (one 21 mL vial of a buffered protein base)
  • Wash Buffer (25X, seven 21 mL vials)
  • Reagent Diluent Concentrate 3 (5X, two 21 mL vials of a 25% Tween 20 solution in PBS).
    For Use as the blocking buffer.


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for DuoSet ELISA Ancillary Reagent Kit 1

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
    Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
    Scientific Reports, 2021;11(1):3802.  2021
  2. GLP-1 receptor agonist ameliorates experimental lung fibrosis
    Authors: J Fandiño, L Toba, LC González-M, Y Diz-Chaves, F Mallo
    Sci Rep, 2020;10(1):18091.  2020


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Reviews for DuoSet ELISA Ancillary Reagent Kit 1

Average Rating: 4.8 (Based on 6 Reviews)

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DuoSet ELISA Ancillary Reagent Kit 1
By Anonymous on 12/04/2020

Used with the Mouse TGF-beta 1 DuoSet ELISA. Performed with precision from one plate to the next. Instructions were complete and easy to follow.

DuoSet ELISA Ancillary Reagent Kit 1
By Anonymous on 10/29/2019

Confusing reagent labels especially blocking buffer

DuoSet ELISA Ancillary Reagent Kit 1
By Anonymous on 08/28/2019

instructions kind of confusing - have the directions are on the ancillary kit and the other are with the antibody kit. Also some of the buffers names need to be clearer and more consistent (e.g. blocking and coating). Results are good though so thats what matters.

DuoSet ELISA Ancillary Reagent Kit 1
By Anonymous on 04/05/2019

DuoSet ELISA Ancillary Reagent Kit 1
By Anonymous on 10/17/2018

DuoSet ELISA Ancillary Reagent Kit 1
By Anonymous on 09/18/2018