DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Specificity
    Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1042E
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    DYKDDDDK epitope tag
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 488‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

Ctrl IC105G
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Rabbit IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC1051G
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