DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC8529G-025
IC8529G-100
Detection of DYKDDDDK Epitope Tag in HEK293 cells by Flow Cytometry.
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Product Details
Citations (4)
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Reviews (1)

DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1042E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
DYKDDDDK epitope tag
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.001-1 ug/106 cells. Sample used for this experiment was HEK293 cells transfected with FLAG, fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of DYKDDDDK Epitope Tag in HEK293 cells by Flow Cytometry. HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8529G) at 0.001 ug/106 cells. To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Product Datasheets

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Product Specific Notices

FLAG® is a registered trademark of Sigma-Aldrich Co. LLC


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. T cells selectively filter oscillatory signals on the minutes timescale
    Authors: Geoff P. O’Donoghue, Lukasz J. Bugaj, Warren Anderson, Kyle G. Daniels, David J. Rawlings, Wendell A. Lim
    Proceedings of the National Academy of Sciences
  2. HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesicles
    Authors: Mitrut, RE;Stranford, DM;Chan, JM;Bailey, MD;Luo, M;Meade, TJ;Wang, M;Leonard, JN;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Extracellular Vesicles, Whole Cells
    Applications: Flow Cytometry
  3. ADR3, a next generation i-body to human RANKL, inhibits osteoclast formation and bone resorption
    Authors: H Qiu, C Hosking, E Rothzerg, A Samantha, K Chen, V Kuek, H Jin, S Zhu, A Vrielink, K Lim, M Foley, J Xu
    The Journal of Biological Chemistry, 2023-01-09;0(0):102889.
    Species: Human
    Sample Types: Whole Cells
  4. Programming Multicellular Assembly with Synthetic Cell Adhesion Molecules
    Authors: AJ Stevens, AR Harris, J Gerdts, KH Kim, C Trentesaux, JT Ramirez, WL McKeithan, F Fattahi, OD Klein, DA Fletcher, WA Lim
    Nature, 2022-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody

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DYKDDDDK Epitope Tag Alexa Fluor® 488‑conjugated Antibody
By Anonymous on 04/13/2023
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human