Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 488‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
No product specific FAQs exist for this product, however you may
Have you used DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.