Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-DYKDDDDK Epitope Tag HRP-conjugated Monoclonal Antibody (Catalog # HAM85291). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
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