DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody.
(2 citations)   
  • Specificity
    Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1042E
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    DYKDDDDK epitope tag
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 647‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529R, filled histogram) or rabbit IgG Alexa Fluor® 647 isotype control antibody (open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
    Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
    Nat Genet, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
    Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
    Nat Genet, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rabbit IgG Alexa Fluor® 647-conjugated Antibody

Ctrl IC1051R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information