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DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
  (3 citations)
(1 Review)     
Datasheet / CoA / SDS
Detection of DYKDDDDK (FLAG<SUP>&#174;</SUP>) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry.

Intracellular Staining by Flow Cytometry

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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
  • Specificity
    Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1042E
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    DYKDDDDK epitope tag
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 647]
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Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 647‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529R, filled histogram) or rabbit IgG Alexa Fluor® 647 isotype control antibody (open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
 Applications
 Sample Type
  1. Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle
    Authors: LJ Albee, HM LaPorte, X Gao, JM Eby, YH Cheng, AM Nevins, BF Volkman, V Gaponenko, M Majetschak
    Open Biol, 2018;8(1):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
    Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
    Nat Genet, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
    Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
    Nat Genet, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rabbit IgG Alexa Fluor® 647-conjugated Antibody

 Ctrl IC1051R
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Average Rating: 1 (Based on 1 review)

Have you used DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody?

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Read what people are saying who have used DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody.

We have 1 review tested in 1 species: Hamster,Human.
We have 1 review tested in 1 application: Live Cell Fluorescence Correlation Spectroscopy .

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Live Cell Fluorescence Correlation Spectroscopy
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Images Ratings Applications Species Reviewed By Date Details
  Poor
  Live Cell Fluorescence Correlation Spectroscopy Hamster,Human Anonymous 03/16/2018
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Summary

Application Live Cell Fluorescence Correlation Spectroscopy
Sample Tested CHO Chinese hamster ovary cell line,HEK293 human embryonic kidney cell line
Species Hamster,Human

Other Experimental Details

Other Experimental Details Diffusion dynamics of FLAG tagged proteinTechnical Service will be following up with the customer

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