DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody
DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 647-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529R, filled histogram) or rabbit IgG Alexa Fluor® 647 isotype control antibody (open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Product Datasheets
Product Specific Notices
FLAG® is a registered trademark of Sigma-Aldrich Co. LLC
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Role for N-glycans and calnexin-calreticulin chaperones in SARS-CoV-2 Spike maturation and viral infectivity
Authors: Q Yang, A Kelkar, A Sriram, R Hombu, TA Hughes, S Neelamegha
Science Advances, 2022;8(38):eabq8678.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle
Authors: LJ Albee, HM LaPorte, X Gao, JM Eby, YH Cheng, AM Nevins, BF Volkman, V Gaponenko, M Majetschak
Open Biol, 2018;8(1):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
Nat Genet, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
Nat Genet, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Used to stain a library of cells expressing protein variants, followed by sorting and sequencing. These high throughput FLAG measurements correlated well with individual Western blots.
Live Cell Fluorescence Correlation Spectroscopy
Diffusion dynamics of FLAG tagged protein
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