Home / DYKDDDDK Epitope Tag / DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody

DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody

IC8529R 3 Citations 2 Reviews Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC8529R-025
IC8529R-100
Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1042E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
DYKDDDDK epitope tag
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of DYKDDDDK (FLAG<SUP>®</SUP>) antibody in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins antibody by Flow Cytometry. View Larger

Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 647-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529R, filled histogram) or rabbit IgG Alexa Fluor® 647 isotype control antibody (open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Product Datasheets

Product Datasheet
COA
SDS

Citations for DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle
    Authors: LJ Albee, HM LaPorte, X Gao, JM Eby, YH Cheng, AM Nevins, BF Volkman, V Gaponenko, M Majetschak
    Open Biol, 2018;8(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
    Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
    Nat Genet, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
    Authors: AR Moore, E Ceraudo, JJ Sher, Y Guan, AN Shoushtari, MT Chang, JQ Zhang, EG Walczak, MA Kazmi, BS Taylor, T Huber, P Chi, TP Sakmar, Y Chen
    Nat Genet, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Rabbit IgG Alexa Fluor® 647-conjugated Antibody

IC1051R
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Reviews for DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody

Average Rating: 3 (Based on 2 Reviews)

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DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody
By Anonymous on 11/03/2020
Application: Flow cytometry and cell sorting Sample Tested: K562 human chronic myelogenous leukemia cell line Species: Human

Used to stain a library of cells expressing protein variants, followed by sorting and sequencing. These high throughput FLAG measurements correlated well with individual Western blots.

Flow cytometry and cell sorting DYKDDDDK Epitope Tag Alexa Fluor® 647-conjugated Antibody IC8529R
DYKDDDDK Epitope Tag Alexa Fluor® 647‑conjugated Antibody
By Anonymous on 03/16/2018
Application: Other Sample Tested: CHO Chinese hamster ovary cell line,HEK293 human embryonic kidney cell line Species: Hamster,Human

Live Cell Fluorescence Correlation Spectroscopy
Diffusion dynamics of FLAG tagged protein

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