Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Simple Western, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1042E
View all DYKDDDDK Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

DYKDDDDK epitope tag

Specificity

Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DYKDDDDK Epitope Tag Antibody

Detection of DYKDDDDK (FLAG®) Epitope Tag antibody by Western Blot.

Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1, N-terminal DYKDDDDK-tagged UCHL5, and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa, 40 kDa, 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of DYKDDDDK (FLAG®) Epitope Tag antibody by Simple WesternTM.

Detection of DYKDDDDK (FLAG®) Epitope Tag by Simple WesternTM.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, loaded at 0.1 mg/mL. Specific band were detected for DYKDDDDK-tagged proteins at approximately 64 and 136 kDa (as indicated) using 1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of DYKDDDDK Epitope Tag in HEK293 cells transfected with FLAG vs Irrelevant HEK293 wild type cells by Flow Cytometry.

HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) at 0.001 ug/106 cells followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for DYKDDDDK Epitope Tag Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 µg/100 µg cell lysate
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, see our available Western blot detection antibodies.

Intracellular Staining by Flow Cytometry

0.001 µg/106 cells
Sample: HEK293 cells transfected with FLAG, fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).

Simple Western

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20

Western Blot

0.1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1, N-terminal DYKDDDDK-tagged UCHL5, and N-terminal DYKDDDDK-tagged RNF20

Reviewed Applications

Read 4 reviews rated 4 using MAB8529 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DYKDDDDK Epitope Tag

Fusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression.

The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).

References

1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796

2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294

3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954

FLAG(R) is a trademark of Sigma-Aldrich.

Alternate Names

FLAG Tag

Additional DYKDDDDK Epitope Tag Products

Product Documents for DYKDDDDK Epitope Tag Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for DYKDDDDK Epitope Tag Antibody

For research use only

Citations for DYKDDDDK Epitope Tag Antibody

Customer Reviews for DYKDDDDK Epitope Tag Antibody (4)

4 out of 5
4 Customer Ratings
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Showing  1 - 4 of 4 reviews Showing All
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  • DYKDDDDK Epitope Tag Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 09/01/2021
    DYKDDDDK Epitope Tag Antibody MAB8529
  • DYKDDDDK Epitope Tag Antibody
    Name: Lucy Quach
    Application: Simple Western
    Sample Tested: FLAG tag protein and transduced cell lines
    Species: Human
    Verified Customer | Posted 11/02/2018
  • DYKDDDDK Epitope Tag Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: HEK293 cell line
    Species: Human
    Verified Customer | Posted 09/29/2017
    1:100 dilution
    NOVUSLOOKSLIKE
    DYKDDDDK Epitope Tag Antibody MAB8529
  • DYKDDDDK Epitope Tag Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: HEK293
    Species: Human
    Verified Customer | Posted 05/22/2017
    HEK293 transiently overexpressing a FLAG-tagged protein were fixed with PFA 4%. MAB8529 antibody was diluted 1:75 and detected with anti-rabbit 488 secondary antibody. Some background, principally in the nucleus. It works well also 1:100.
    DYKDDDDK Epitope Tag Antibody MAB8529

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