DYKDDDDK Epitope Tag Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8529
Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

DYKDDDDK Epitope Tag Antibody Summary

Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1042E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
DYKDDDDK epitope tag
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunoprecipitation
1-4 µg/100 µg cell lysate
HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, see our available Western blot detection antibodies.
Intracellular Staining by Flow Cytometry
0.001 µg/106 cells
HEK293 cells transfected with FLAG, fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of DYKDDDDK (FLAG<SUP>®</SUP>) Epitope Tag antibody by Western Blot. View Larger

Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1, N-terminal DYKDDDDK-tagged UCHL5, and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa, 40 kDa, 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of DYKDDDDK (FLAG<SUP>®</SUP>) Epitope Tag antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of DYKDDDDK (FLAG®) Epitope Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, loaded at 0.1 mg/mL. Specific band were detected for DYKDDDDK-tagged proteins at approximately 64 and 136 kDa (as indicated) using 1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Intracellular Staining by Flow Cytometry View Larger

Detection of DYKDDDDK Epitope Tag in HEK293 cells transfected with FLAG vs Irrelevant HEK293 wild type cells by Flow Cytometry. HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) at 0.001 ug/106 cells followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Citations for DYKDDDDK Epitope Tag Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Identification of the Functional Autophagy-Regulatory Domain in HCLS1-Associated Protein X-1 That Resists Against Oxidative Stress.
    Authors: Li YL, Cai WF, Wang L et al.
    DNA Cell Biol
  2. N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release
    Authors: PR Kamble, SR Patkar, AA Breed, BR Pathak
    Archives of biochemistry and biophysics, 2021-11-10;714(0):109084.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models
    Authors: WH Liu, K Völse, D Senft, I Jeremias
    Scientific Reports, 2021-06-16;11(1):12649.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
    Authors: W Zhang, G Yin, H Zhao, H Ling, Z Xie, C Xiao, Y Chen, Y Lin, T Jiang, S Jin, J Wang, X Yang
    Cell Death & Disease, 2021-01-20;12(1):102.
    Species: Human
    Sample Types: Cell Lystates
    Applications: Immunoprecipitation

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Reviews for DYKDDDDK Epitope Tag Antibody

Average Rating: 4 (Based on 2 Reviews)

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DYKDDDDK Epitope Tag Antibody
By Anonymous on 09/01/2021
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

DYKDDDDK Epitope Tag Antibody
By Lucy Quach on 11/02/2018
Application: Simple Western Sample Tested: FLAG tag protein,transduced cell lines Species: Human