NleL (Non-Lee-encoded effector Ligase, also known as espX7) is an effector protein translocated into host cells via the type III secretion system of Escherichia coli O157:H7, a widespread pathogen causing severe foodborne diseases. Although the biological relevance of NleL is presently unknown, recent studies clearly demonstrate that this protein is functionally related to the HECT (Homologous to E6AP Carboxy Terminus) class E3 Ubiquitin Ligases of higher eukaryotes. NleL shows no primary sequence homology to any eukaryotic proteins, but biochemical and structural studies revealed both N-terminal and C-terminal lobes connected by a flexible linker, and a catalytic cysteine that accepts ubiquitin from E2 carrier proteins, both hallmarks of HECT domain ligases. NleL catalyzes the formation of mixed K6 and K48-linked unanchored polyubiquitin chains. This recombinant protein contains amino acid residues 170-782 (UniProt Q8X5G6 numbering).
E. coli O157:H7 NleL Catalytic Domain Protein, CF
R&D Systems | Catalog # E3-250
Key Product Details
Source
Accession Number
Applications
Product Specifications
Source
Purity
Predicted Molecular Mass
Activity
Escherichia coli O157:H7 recombinant NleL Catalytic Domain is a bacterial HECT class Ubiquitin Ligases (E3) that catalyzes the formation of mixed K6 and K48-linked unanchored polyubiquitin chains. Reaction conditions will need to be optimized for each specific application. We recommend an initial E. coli O157:H7 recombinant NleL concentration of 0.1-0.5 μM.
Formulation, Preparation, and Storage
E3-250
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
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| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: NleL
References
- Lin D.Y., et al. (2011) J. Biol. Chem. 286: 441-449.
- Lin D.Y., et al. (2012) Proc. Natl. Acad. Sci. 109: 1925-1930.
Long Name
UniProt
Product Documents for E. coli O157:H7 NleL Catalytic Domain Protein, CF
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