EB2 Antibody (KT52) - BSA Free

Novus Biologicals | Catalog # NBP3-23289

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # KT52

Format

BSA Free
View all EB2 Primary Antibodies »

Product Specifications

Immunogen

Clone KT52 was generated from a GST fusion protein containing human EB2. This sequence is highly conserved in rat and mouse EB2. [UniProt# Q15555]

Specificity

The antibody detects a 36 kDa protein corresponding to the molecular mass of EB2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB2 by immunocytochemistry in HeLa and A431 cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for EB2 Antibody (KT52) - BSA Free

EB2 Antibody (KT52)

Western Blot: EB2 Antibody (KT52) [NBP3-23289] -

Western Blot: EB2 Antibody (KT52) [NBP3-23289] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.
EB2 Antibody (KT52)

Immunocytochemistry/Immunofluorescence: EB2 Antibody (KT52) [NBP3-23289] -

Immunocytochemistry/Immunofluorescence: EB2 Antibody (KT52) [NBP3-23289] - Immunocytochemical labeling of EB2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-EB2 (NBP3-23289) (right). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.

Applications for EB2 Antibody (KT52) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EB2

EB2 is encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Alternate Names

APC-binding protein EB2, EB1, EB2APC-binding protein EB1, End-binding protein 2, microtubule-associated protein, RP/EB family, member 2, RP1microtubule-associated protein RP/EB family member 2, T-cell activation protein, EB1 family

Gene Symbol

MAPRE2

Additional EB2 Products

Product Documents for EB2 Antibody (KT52) - BSA Free

Certificate of Analysis

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Product Specific Notices for EB2 Antibody (KT52) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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