EB2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84926

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EB2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFYDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKSSPAAKPGSTPSRPSSAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EB2 Antibody - BSA Free

Western Blot: EB2 Antibody [NBP1-84926]

Western Blot: EB2 Antibody [NBP1-84926]

Western Blot: EB2 Antibody [NBP1-84926] - Analysis in human cell lines A-549 and A-431 using Anti-MAPRE2 antibody. Corresponding MAPRE2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: EB2 Antibody [NBP1-84926]

Western Blot: EB2 Antibody [NBP1-84926]

Western Blot: EB2 Antibody [NBP1-84926] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926] - Staining of human cerebral cortex, colon, liver and testis using Anti-MAPRE2 antibody NBP1-84926 (A) shows similar protein distribution across tissues to independent antibody NBP1-84927 (B).
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926] - Analysis in human cerebral cortex and testis tissues. Corresponding MAPRE2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926]

Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84926] - Staining of human colon shows moderate cytoplasmic positivity in peripheral nerve / ganglion.

Applications for EB2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EB2

EB2 is encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Alternate Names

APC-binding protein EB2, EB1, EB2APC-binding protein EB1, End-binding protein 2, microtubule-associated protein, RP/EB family, member 2, RP1microtubule-associated protein RP/EB family member 2, T-cell activation protein, EB1 family

Gene Symbol

MAPRE2

Additional EB2 Products

Product Documents for EB2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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